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. 2018 Apr 27;9(5):460. doi: 10.1038/s41419-018-0543-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Schematic representation of injured spinal cord. Transverse sections were prepared from 6 mm rostral b, or adjacent to the lesion site (F). b The number of MPO-positive neutrophils was decreased in the injured spinal cord of CI-994-treated mice at 1 day and 3 days after SCI. dpi: days post injury. Scale bar: 200 μm. c Dot plots of isolated immune cells in the spinal cord, gated for live cell analysis. d Representative cytometry data for neutrophils (Ly6C⁺/Ly6G⁺ cells) in the injured spinal cord 3 days after SCI. e Quantitative data for accumulated neutrophils in the injured spinal cord up to 7 days after SCI. f Numbers of CD68 (ED-1)-positive activated microglia/macrophages were not altered in the injured spinal cord of CI-994-treated mice. Scale bar: 200 μm in low-magnification images, 100 μm in high magnification images. g Representative cytometry data for macrophages (Ly6C⁺/CD11b⁺ cells) in the injured spinal cord at the indicated days after SCI. hQuantitative data for accumulated macrophages in the injured spinal cord up to 7 days after SCI. Results are presented as the mean ± SE (n = 3–6). *p < 0.05, Two-way repeated-measures ANOVA. SCI: spinal cord injury; MPO: myeloperoxidase