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. 2018 Apr 26;8:6627. doi: 10.1038/s41598-018-25070-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Survival rate after transplantation by preservation method. Limbs were preserved in chambers filled with CO + O₂ for 3, 7, and 10 days, and CO + N₂, or N₂ + O₂ for 3 days under high pressure. Negative control was achieved by wrapping limbs in saline-moistened gauze (SMG) for 3 days at 4 °C. For the positive control, non-preserved (NP) limbs were used from donor rats.