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. 2018 Apr 26;8:6554. doi: 10.1038/s41598-018-24919-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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eMSC transduction and survival of eMSC on PA + G mesh in NSG mice. (A) cultured mCherry transduced eMSC showing red fluorescence, (B) more than 95% of transduced and cultured eMSC were mCherry⁺ by flow cytometry and about 40% of this population were SUSD2⁺. Representative trace of n = 6 patient samples, (C) PA + G mesh seeded and cultured with eMSC. (D,E) mCherry⁺ eMSC were observed 3 and (F,G) 7 days post-implantation around the mesh filaments in immunocompromised NSG mice. Arrows, representative mCherry⁺ eMSC; m, mesh filament; g, gelatin. Scale Bars 100 µm.