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. 2018 Apr 26;8:6554. doi: 10.1038/s41598-018-24919-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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M2 macrophage quantification around implanted mesh in C57BL/6 and NSG mice. CD206 M2 macrophage (white) co-localized with the pan macrophage F4/80 marker (green) in (A) C57BL/6 mice and in (B) NSG mice. The ratio of CD206⁺ macrophages to total F4/80 macrophages (MQ) in the first 100 µm increment around mesh filaments in (C) C57BL/6 and in (D) NSG mice. Data are mean ± SEM of n = 6 animals/group and were analyzed by two-way ANOVA. *P < 0.05. Arrows show representative CD206⁺F4/80⁺ macrophages within and outside the 100 µm increment around PA + G mesh, m, mesh filament; g, gelatin. Scale Bars 100 µm.