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Details of antibodies used in immunostaining.
| Primary antibody | Isotype | Supplier | Dilution(Concentration) | Secondary Ab | Concentration | Supplier |
|---|---|---|---|---|---|---|
| CCR7 | Rabbit monoclonal | Abcam | 1/200 (1 µg/ml) | Donkey anti rabbit IgG | 1/500 | Life Technologies |
| CD206 | Rat IgG2a- Alexa Fluor 647 conjugated | Biolegend | 1/100 (5 µg/ml) | — | — | — |
| F4/80 | Rat IgG2b | BioRad | 1/200 (1 µg/ml) | Chicken anti rat IgG | 1/500 | Life Technologies |
