Figure 1.

Vasoinhibin binds integrins 0, 10, 100 nM biotinylated proteins were added to 96-well plate coated with integrin beta1 (A), integrin alpha5 beta1 (B), alpha1 beta1 (C) or alphaV beta3 (D) at 100 ng/well concentration. Absorbance was measured 3 h after addition of biotinylated proteins. (A) Absorbance of biotinylated Vi incubated with integrin beta1 was significantly higher than that of biotinylated FN (P < 0.01). (B) Absorbance of biotinylated Vi incubated with integrin alpha5 beta1 was significantly higher than that of biotinylated FN (P < 0.01). (C) Absorbance of biotinylated Vi incubated with integrin alpha1 beta1 was significantly higher than that of BLANK (P < 0.01). (D) Absorbance of biotinylated Vi incubated with integrin alphaV beta3 was significantly higher than that of BLANK (P < 0.01). Data represent mean ± s.d. of 3 independent experiments, double asterisks indicate significant differences (P < 0.01).