Figure 3.

Integrin antibody inhibits an apoptotic effect of vasoinhibin incubating HUVEC with Vi for 24 h increases the TUNEL-positive cell rate. Integrin beta1 antibody (A, B) or alpha5 beta1 antibody (C, D) was added to HUVEC before incubation with Vi. (A and C) Immunofluorescence images showed that the integrin antibodies decreased TUNEL-stained cell (green). Nuclei are stained blue. Scale bar indicates 100 µm. (B) Integrin beta1 antibody decreased the TUNEL-positive cell rate induced by Vi (P < 0.01). (D) Integrin alpha5 beta1 antibody also decreased TUNEL-positive cell rate induced by Vi (P < 0.01). Data represent mean ± s.d. of 3 independent experiments, double asterisks indicate significant differences (P < 0.01).