Figure 6.

Adrenocortical cells cultured in MGPM and CM were stained for expression of adrenal-specific (SF1) and stem cell morphogenic signalling markers (GLI1, DAX1) and examined under fluorescence microscopy. Nuclei were stained with DAPI (blue, right hand column). Cells cultured in MGPM showed positive staining for SF1, GLI1 and DAX1. The cell population seeded in CM demonstrated strong SF1 expression but weak GLI with negative DAX1 staining. (Magnification ×200.)