Figure 8.

It demonstrates the multilineage differentiation capacity of adrenal cortex-derived cells. Adrenal cells cultured in MGPM were processed into cell pellets and induced with chondrogenic differentiation medium for 21 days. One of three cell pellets (from 3 individual patients) demonstrated weak staining with Safranin O solution, signifying the presence of GAG (A) but two of the remaining 2 showed negative staining (B). Only cells seeded in MGPM survived adipogenic differentiation medium and differentiated into adipogenic cells in abundance, as indicated by cellular accumulation of lipid-rich vacuoles (C), which stained with Oil Red O (D). Cell population seeded in either CM or MGPM (E) or CM (F) differentiated into osteogenic lineage at day-21 with enhancement of alkaline phosphatase activity as shown by the positive staining for Alizarin S.