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. 2018 Apr 20;9:178. doi: 10.3389/fendo.2018.00178

Figure 2.

Figure 2

Assessment of IGF1R as an insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) mRNA target in Ewing sarcoma (ES) cells. (A) Quantitative real-time PCR analysis of IGF2BP3-associated mRNAs isolated from the cytoplasmic extracts of A673 cells by immunoprecipitation using an anti-IGF2BP3 antibody. Non-immune goat IgG was used as a negative control. ABCG2 was used as positive control. Columns represent the mean values of two independent experiments, in which samples were run in triplicates, and the bars represent the SE. *p < 0.05, **p < 0.01, Student’s t-test. (B) mRNA expression levels of IGF2BP3 and IGF1R in IGF2BP3-depleted or empty vector-transfected (shCTR) A673 ES cells. GAPDH was used as a housekeeping gene. Columns represent the mean values of at least two independent experiments, in which samples were run in triplicates, and the bars represent the SE. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA with respect to shCTR. (C) Western blotting showing IGF2BP3 and IGF1R expression in IGF2BP3-depleted or empty vector-transfected (shCTR) A673 (left) or TC-71 (right) ES cells. GAPDH was used for normalization. Data from one experiment representative of three.