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. 2018 Apr 20;9:178. doi: 10.3389/fendo.2018.00178

Figure 4.

Figure 4

Insulin receptor (IR) signaling in response to insulin-like growth factor 2 (IGF2) mRNA-binding protein 3 (IGF2BP3)-mediated IGF1R regulation. (A,B) Western blotting depicting the expression of IR and phosphorylation of Akt and ERK 1/2 in IGF2BP3-depleted or empty vector-transfected (shCTR) (A) A673 or (B) TC-71 Ewing sarcoma (ES) cells. GAPDH, Lamin B, and total proteins were used for normalization purposes. Data from one experiment representative of three. (C,D) mRNA level of IGF1 and IGF2, expressed as 2−ΔΔCt, in IGF2BP3-depleted or empty vector-transfected (shCTR) (C) A673 or (D) TC-71 cells. Columns represent the mean values of at least two independent experiments, in which samples were run in triplicates, and the bars represent the SE. *p < 0.05, **p < 0.01, one-way ANOVA compared with shCTR.