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. 2018 Apr 20;12:108. doi: 10.3389/fncel.2018.00108

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Copyright © 2018 Lin, Li, Winters, Liu and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Akt pathway inhibitor MK2206 abolished the protective effect of artemisinin against glutamate insult in HT-22 cells. (A) Calcein-AM cell viability assay demonstrated that MK2206 attenuated the protective effects of artemisinin against glutamate-induced cell death. (B,C) Annexin V/PI flow cytometry analysis showed that MK2206 abolished the protective effect of artemisinin against glutamate-induced apoptosis. (D,E) Western blots analysis indicated that MK2206 attenuated artemisinin-induced increased of pAkt/Akt and Bcl-2/Bax ratio. (F,G) Western blots demonstrated that MK2206 abolished artemisinin-induced decrease of cleaved caspase-3 and cleaved PRAP expression in HT-22 cells. The antibodies in Figures 6E,F were incubated in the same gel. Therefore, they had the same reference. ^#p < 0.05 vs. CLUT group, *p < 0.05 vs. GLUT+ART group.