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. 2018 Apr 20;9:135. doi: 10.3389/fgene.2018.00135

FIGURE 3.

FIGURE 3

Cell proliferation and cisplatin sensitivity after the overexpression or knockdown of TIMP3 in Saos2-lung cells. (A–D) Cisplatin sensitivity of transfected-Saos2-lung cells at 24, 48, 72, and 96 h. (E) Cell proliferation in transfected-Saos2-lung cells without cisplatin. (F) Flow cytometry assessment of apoptosis in transfected-Saos2-lung cells without or with cisplatin treatment. (G) Apoptosis rates of transfected-Saos2-lung cells with or without cisplatin treatment. (H) Hoechst staining of transfected-Saos2-lung after cisplatin treatment for 24 h. (I) The quantification of hoechst staining. The results are expressed as the ratio of p-Akt to t-Akt. Data are presented as the means ± SD. ∗P < 0.05 between the Saos2-lung and siRNA groups, #P < 0.05 between the Saos2-scramble and Saos2-lung-TIMP3 groups, separately, in a cell proliferation assay. ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05. All data were obtained from at least three independent experiments.