Table 1.
Effect of the absence of each macronutrient in the Hoagland solution on the homoeologous CO frequency of T. aestivum cv. Chinese Spring Tazip4-B2 ph1b mutant-rye hybrids.
| No. of cell examined | Univalents | Rod bivalents | Ring bivalents | Trivalents | Chiasma frequency | ||
|---|---|---|---|---|---|---|---|
| Mean ± SE (Range) | Mean ± SE (Range) | Mean ± SE (Range) | Mean ± SE (Range) | Mean ± SE (Range) | Mean ± SE (Range) | ||
| Single chiasma | Double chiasmata | ||||||
| Hoagland solution | 109 | 12.36 ± 0.21c (7–18) |
4.39 ± 0.14a (2–8) |
2.57 ± 0.10abc (0–5) |
0.58 ± 0.07a (0–3) |
10.74 ± 0.16a (7–14) |
12.03 ± 0.22a (7–18) |
| Water alone | 108 | 15.93 ± 0.16a (12–20) |
4.03 ± 0.13ab (1–7) |
1.68 ± 0.09bc (0–4) |
0.22 ± 0.04bc (0–1) |
7.91 ± 0.13d (5–10) |
8.15 ± 0.15d (5–12) |
| Hoagland solution - NH4 H2PO4 | 92 | 13.61 ± 0.22b (8–17) |
3.83 ± 0.14b (2–8) |
2.78 ± 0.13a (0–6) |
0.39 ± 0.06ab (0–2) |
10.25 ± 0.18b (7–14) |
11.03 ± 0.23b (7–17) |
| Hoagland solution - KNO3 | 93 | 13.86 ± 0.22b (6–18) |
4.34 ± 0.16a (2–8) |
2.35 ± 0.12abc (0–5) |
0.25 ± 0.05c (0–2) |
9.57 ± 0.18c (7–16) |
9.96 ± 0.19c (7–16) |
| Hoagland solution - Ca(NO3)2·4H2O | 102 | 13.84 ± 0.20b (9–18) |
4.14 ± 0.13ab (1–7) |
2.50 ± 0.09c (0–4) |
0.29 ± 0.05bc (0–2) |
9.79 ± 0.15c (7–14) |
10.12 ± 0.17c (7–15) |
| Hoagland solution - Mg SO4·7H2O | 90 | 15.63 ± 0.24a (10–20) |
4.12 ± 0.16ab (1–8) |
1.68 ± 0.13ab (0–4) |
0.26 ± 0.06bc (0–2) |
8.09 ± 0.20d (5–12) |
8.62 ± 0.22d (5–13) |
| P-value | 0.0000 | 0.0538 | 0.0592 | 0.0516 | 0.0000 | 0.0000 | |
Frequencies of univalents, bivalents, trivalents and chiasma frequency (single and double chiasmata) were scored at meiotic metaphase I in Tazip4-B2 ph1b mutant-rye hybrids. Values in parenthesis indicate range of variation between cells. P < 0.05 indicates significant differences according to LSD test. Different letters indicate significant differences between treatments.