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. 2018 Apr 20;9:696. doi: 10.3389/fimmu.2018.00696

Figure 3.

Figure 3

Lamin A/C overexpression induces proinflammatory cytokine expression in macrophages. (A–C) Raw 264.7 cells were transfected with GFP or GFP-Lmna. After 24 h, transfected cells were cultured in the absence or presence of 100 ng/ml LPS for 6 h. (A) Quantitative real-time RT-PCR (qRT-PCR) analysis of Lmna and Lmnb1 in GFP or GFP-Lmna-transfected cells. (B) qRT-PCR analysis of Il6, Tnf, Ccl2, and Nos2 in GFP or GFP-Lmna transfected cells. (C) ELISA of IL-6 in supernatant of Raw 264.7 cells transfected with GFP or GFP-Lmna in the absence of LPS. (D) Characterization of bone marrow-derived macrophages (BMDMs) transfected GFP-Lmna. qRT-PCR analysis of Lmna and Lmnb1 (left) and Il6, Tnf, Ccl2, and Nos2 in GFP or GFP-Lmna transfected BMDMs (right). Error bars represent SEM. *p < 0.05, ***p < 0.001.