Skip to main content
. 2018 Apr 20;9:696. doi: 10.3389/fimmu.2018.00696

Figure 4.

Figure 4

Lamin A/C increases NF-κB activity via nuclear translocation of p65/Rel A, a NF-κB complex subunit. (A) qRT-PCR analysis of Nfkb1 and Rela in Raw 264.7 cells transfected with either GFP or GFP-Lmna. (B) NF-κB luciferase activity in Raw 264.7 cells co-transfected with GFP or GFP-Lmna together with pNF-κB-Luc, a luciferase reporter construct that has κB responsible elements in the promoter. Luciferase activities were normalized by those in cells co-transfected with a non-reporter plasmid, pRL-SV40. (C) Immunofluorescence images of bone marrow-derived macrophages transfected with GFP (Mock) or GFP-Lmna (Lmna) and quantitation for cells with nuclear NF-κB p65/Rel A. Cells with nuclear NF-κB were defined based on the presence of nuclear signal and obvious lack of cytoplasmic signal as shown in the representative images (Scale bar: 5 μm). (D) Immunoblots of lysates from transfected Raw 264.7 cells with anti-Lamin A/C (top), anti-IκBα (middle), or anti-actin (bottom). Arrowhead marks GFP fusion lamin A protein size.