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. 2018 Apr 20;9:696. doi: 10.3389/fimmu.2018.00696

Figure 5.

Figure 5

Depletion of lamin A/C suppresses proinflammatory gene activation upon LPS treatment in macrophages. (A–E) Analyses of peritoneal macrophages from control and myeloid cell-specific Lmna KO mice (MKO) mice. (A) PCR genotyping of peritoneal macrophages from WT control (CON, Lmnaflox/flox) and MKO (LysM-Cre; Lmnaflox/flox) mice. Arrowheads mark Lmnaflox (uncleaved) and LmnaΔ (cleaved) alleles. (B) qRT-PCR analysis of Lmna and Lmnb1 in peritoneal macrophages isolated from CON and MKO mice. (C) Immunoblots of lysates from peritoneal macrophages for lamin A/C (top), IκBα (middle), or actin (bottom). (D,E) Peritoneal macrophages were isolated from control and MKO mice and then treated with vehicle or 10 ng/ml LPS for 2 h. (D) qRT-PCR analysis of Il6, Tnf, and Ccl2 in peritoneal macrophages isolated from CON and MKO mice. (E) Level of IL-6 in supernatant of CON and MKO peritoneal macrophages treated with vehicle or 10 ng/ml LPS for 6 h. (F) Plasma TNFα and MCP-1 levels after i.p. injection of LPS (20 mg/kg BW) were measured in CON and MKO mice (n = 6 per group). Error bars represent SEM. *p < 0.05, **p < 0.01.