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. 2018 Apr 20;11:120. doi: 10.3389/fnmol.2018.00120

FIGURE 1.

FIGURE 1

Morphine protected SH-SY5Y human neuroblastoma cells against 6-OHDA–induced cell apoptosis. (A) RT-qPCR analysis of the mRNA expression of CYP2D6, CYP3A4 and CYP3A5 following 6-OHDA treatment. ∗∗∗P < 0.001 vs. control. (B) The morphology of SH-SY5Y cells under phase contrast microscope. Scale bars: 50 μm. (C) Analysis of cell viability using the MTT assay in SH-SY5Y cells treated with 6-OHDA. n = 6–8 wells/group. (D) The effect of morphine on SH-SY5Y cells for 24 h by MTT assay. n = 7–8 wells/group. (E) The effect of pretreatment of low-dose morphine (1–100 μM) against the cytotoxicity induced by 6-OHDA (100 μM) in SH-SY5Y cells by MTT assay. ∗∗P < 0.01, ∗∗∗P < 0.001 vs. control group, ###P < 0.001 vs. 6-OHDA-treated group, n = 7–8 wells/group. (F,G) Flow cytometry was used to detect 6-OHDA-induced apoptosis with or without morphine. ∗∗P < 0.01, ∗∗∗P < 0.001 vs. 6-OHDA-treated group. All data are expressed as mean ± SEM.