FIGURE 2.

Morphine suppressed 6-OHDA-induced ER stress through activation of UPR. (A,B) Morphine induced UPR in SH-SY5Y cells. Protein levels of GRP78, p-IRE1α, IRE1α, p-PERK, PERK, ATF6, CHOP and Tubulin in SH-SY5Y cells were analyzed (A) and quantified (B) by western blot. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001 vs. control. (C,D) Protein levels of GRP78, CHOP and Actin in SH-SY5Y cells were analyzed (C) and quantified (D) by western blot in the indicated groups. ^∗P < 0.05, ^∗∗P < 0.01 vs. control and ^#P < 0.05, ^##P < 0.01 vs. 6-OHDA-treated group. All data are expressed as mean ± SEM.