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. 2018 Apr 20;9:753. doi: 10.3389/fmicb.2018.00753

FIGURE 2.

FIGURE 2

Effect of KP treatment on the efficiency of T. gondii infection in Vero cells, as measured by quantitative real-time PCR. Vero cells were infected with T. gondii in absence (control), and in presence of KP (50, 100, and 200 μg/mL), or the scrambled peptide SP (200 μg/mL). After 3 h (T0), the medium was replaced with fresh RPMI (2% FBS) without peptides. At 24, 48, or 72 h post-infection, the amount of T. gondii DNA in each sample was determined targeting a 94-bp internal fragment in a 529-bp repeat element of the parasite genome (Accession No.: AF146527). DNA amount is expressed as relative quantity in comparison to DNA obtained at T0. Data reported are the mean values ± SD from three replicate wells (∗∗∗P < 0.001 compared to control, as determined by one-way ANOVA with Tukey post hoc test).