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. 2018 Apr 20;9:753. doi: 10.3389/fmicb.2018.00753

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Copyright © 2018 Giovati, Santinoli, Mangia, Vismarra, Belletti, D’Adda, Fumarola, Ciociola, Bacci, Magliani, Polonelli, Conti and Kramer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of KP treatment on phosphatidylserine exposure in extracellular T. gondii tachyzoites. (A) Percentage values of Annexin-V⁺/7-AAD^- parasites after the indicated periods of incubation with KP at 50 and 100 μg/mL and medium alone (control). Data are expressed as mean values ± SD from at least three independent experiments. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, vs. control; ^##P < 0.01, ^###P < 0.001, vs. treatment with KP at 50 μg/mL; assessed by one-way ANOVA with Tukey post hoc test. (B) Representative two dimensional dot plots obtained after incubation of tachyzoites for 48 h with KP or medium alone (control).