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. 2018 Apr 20;9:753. doi: 10.3389/fmicb.2018.00753

FIGURE 4.

FIGURE 4

Effect of KP treatment on DNA fragmentation in extracellular T. gondii tachyzoites. DNA strand breaks were detected with an in situ TUNEL assay and analyzed by flow cytometry. (A) Percentages of TUNEL positive cells detected in freshly isolated tachyzoites (FI, white bar) and in tachyzoites treated with KP at 50 (gray bars) or 100 μg/mL (black bars) for the indicated periods of time. Percentages of TUNEL positive cells in tachyzoites incubated with medium alone at each time point (non-treated controls) are reported as an average (NT, lined bar). Data are expressed as mean ± SD from two independent experiments, and compared with Student’s t-test to NT value (∗P < 0.05, ∗∗∗P < 0.001). (B) Representative TUNEL fluorescence histograms of T. gondii obtained after treatment with KP at 50 μg/mL. Solid histogram: freshly isolated tachyzoites; open histogram: tachyzoites after 24 or 48 h of treatment.