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. 2018 Apr 20;9:753. doi: 10.3389/fmicb.2018.00753

FIGURE 6.

FIGURE 6

Effect of treatment with KP at 50 μg/mL on TMRM loading in extracellular T. gondii tachyzoites in a time-lapse assay. Confocal images of viable parasites labeled with TMRM before (A) and after incubation with the peptide for 10 min (B), 1 h (C), and 2 h (D). The same field is shown. Signal of TMRM and transmitted light images are merged to highlight both TMRM distribution and tachyzoites morphology. Arrowheads in (A) indicate tachyzoites with stained mitochondria. Bar: 5 μm. The inset in (A) is presented at higher magnification in (E) (TMRM fluorescence) showing the structure of T. gondii mitochondrion, and the position of the mitochondrion into the cell (F, merged image). The same inset after 3 h of incubation with the peptide is presented, showing a low residual TMRM fluorescence localized outside the mitochondrion (G) and prominent alterations of tachyzoites morphology (H, merged image). Bar: 2 μm.