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. 2018 Apr 26;8:6622. doi: 10.1038/s41598-018-24585-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RTS,S Amino Acid Changes and Positions. The 84 amino acids (positions 288–371) comprising the C-terminal amplicon are represented by columns in the bar-chart. The percentage of samples sharing the 3D7 amino acid are represented in pale yellow. Non-3D7 amino acid alternatives are represented in descending order of frequency in dark blue, red, light blue, or orange. Below the bar-chart, the 3D7 amino acid sequence is shown, with positions corresponding to the coordinates above. The substitutions at each of the 84 positions are enumerated below the 3D7 sequence.