Fig. 5.

Bcl11b binds at the Gata3 locus and downstream and at the Il4 HS IV silencer but not at the Th2-LCR or Th2-cytokine promoters in CD4⁺ T-cells. a The top two Bcl11b binding motifs discovered using MEME-ChIP motif analysis software from Bcl11b ChIP-seq of CD4⁺ T-cells from WT mice infected (n = 2) with H. polygyrus bakeri. b Density distribution of Bcl11b binding relative to the nearest transcription start site (TSS). Data obtained from Genomic Regions Enrichment of Annotations Tool (GREAT). c–e Integrative Genomics Viewer visualizations of Bcl11b ChIP-Seq tracks at the Th2 cytokine locus (c, Il4 HS IV silencer is highlighted), Gata3 (d intronic regions are highlighted), and (e conserved non-coding binding sites downstream of Gata3 are highlighted) from naive (n = 2) (c) or helminth-infected (n = 2) (c–e) mice. ChIP-Seq track scale bar normalized to sequences per million reads (SPMR)