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. 2018 Apr 26;8:6586. doi: 10.1038/s41598-018-24633-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Elovl6 knockdown leads to lipid accumulation. (a) The lipid metabolism-related genes were analyzed using real-time RT-PCR after 48 h of infection. (b) The lipid accumulation in cells was detected using the BODIPY stain. Scale bar = 100 μm; original magnification, 200× . Cell numbers for the three groups were counted. (c,d) After 24 h of infection, the cells were treated with palmitate (c) or stearate (d) for 72 h. Cell viability was measured with the WST-8 assay. Data are mean ± S.E.M.