Figure 5.

Knockdown of Elovl6 expression decreases tumor growth in mice. (a) Mice were inoculated subcutaneously with ML-1-shElovl6 or control cells. Tumor volumes were monitored twice a week. (b) Tumor-bearing mice were treated intratumorally with 10⁷ or 10⁸ PFU adenoviruses on days 10 and 16. (c) Kaplan-Meier survival curves for each group of mice. Statistical differences were analyzed with the log-rank test. (d,e) Ki67-positive cells (d) and gap junction protein Cx32 (e) were examined in the Elovl6-knockdown tumor samples on day 14. Scale bar = 50 μm (d) and 40 μm (e); original magnification, 200× (d) and 400× (e).