Table 1. Summary of the used indirect immunofluorescence protocols.

Primary antibody	Dilution/incubation time	Secondary antibody
E-cadherin (36/E-cadherin BD Bioscience)	1:500/1 h RT	Goat anti-mouse FITC-conjugated (Jackson Immuno research) 1:200/1 h RT
Occludin (Invitrogen)	1:100/overnight 4°C	Goat anti-rabbit Alexa Fluor 488 (Molecular Probes) 1:100/1 h RT
Desmocollin-2 (Progen)	1:250/1 h RT	Goat anti-rabbit Alexa Fluor 488 (Molecular Probes) 1:100/1 h RT