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. 2018 Apr 20;9:196. doi: 10.3389/fphar.2018.00196

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Copyright © 2018 Horenstein, Chillemi, Zini, Quarona, Bianchi, Manfredini, Gambari, Malavasi and Ferrari.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of P1 receptors in normal and hypoxic conditions. Comparative cytofluorometric analysis of A₁R, A_2AR and A_2BR expression in CIK cells stained with primary antibodies to various P1 receptors and detected with fluorescein (FITC)-conjugated secondary antibodies as reported in Materials and Methods. To compare data obtained in hypoxia and normoxia we used unpaired nonparametric test, two-tailed Mann-Whitney for GraphPad Prism 6. Circles, normoxia; squares, hypoxia. Patients are as following: PT1, red; PT2, black; PT3, blue; PT4, pink; PT5, green. Reported data are expressed as mean values ± SD.