Figure 6.

Worms Expressing Non-phosphorylatable LMN-1 and SUN-1 Proteins Have a Synthetic Meiotic Phenotype Consisting of Chromosome Interlocks and Aberrant Chromatin Configurations at Diakinesis

(A) Representative images of diakinesis chromosomes from the wild-type and the [gfp::lmn-1^S8A];[sun-1^S10A::gfp] double mutant expressing non-phosphorylatable LMN-1 and SUN-1, stained with DAPI and HTP-3. Filled arrowheads indicate smaller non-bivalent DAPI structures (univalents or fragments); the open arrowhead indicates fused chromatin masses. Scale bars, 5 μm.

(B and B′) Quantification of DAPI-stained bodies in -1 and -2 oocytes from control and [gfp::lmn-1^S8A];[sun-1^S10A::gfp] mutants. [gfp::lmn-1];[sun-1::gfp], n = 93 oocytes; [gfp::lmn-1];[sun-1^S10A::gfp], n = 54 oocytes; [sun-1^S10A::gfp], n = 79 oocytes; [gfp::lmn-1^S32,403A];[sun-1^S10A::gfp], n = 92 oocytes; [gfp::lmn-1^S8A];[sun-1^S10A::gfp], n = 77 oocytes; [gfp::lmn-1];[sun-1::gfp] ced-3, n = 70 oocytes; [sun-1^S10A::gfp] ced-3, n = 67 oocytes; [gfp::lmn-1^S8A];[sun-1^S10A::gfp] ced-3, n = 81 oocytes; [gfp::lmn-1];[sun-1::gfp] spo-11(ok79), n = 73 oocytes; [gfp::lmn-1^S8A];[sun-1^S10A::gfp] spo-11(ok79), n = 74 oocytes.

(C) FISH analysis of the wild-type and [gfp::lmn-1^S8A];[sun-1^S10A::gfp] using a probe for the 5S rDNA locus (chromosome V). Images show a region within the last 10 rows of pachytene nuclei. Arrowheads indicate unpaired FISH signals. Scale bars, 5 μm.

(D) Upper panels: super-resolution Airyscan images showing the localization of axial (HTP-3) and central (SYP-1) SC components in chromosome spreads of control and [gfp::lmn-1^S8A];[sun-1^S10A::gfp] mutant pachytene nuclei. Half nuclei are projected for these images. Lower panel: false color labeling of the six paired homologous chromosomes for better visualization. Arrowheads indicate chromosomal interlocks in nuclei with complete synapsis. Scale bar, 3 μm.