Figure 5.
Accumulation in tobacco leaves of PR proteins determined by immunodetection 48 h after infiltration with laminarin. Leaves were infiltrated at discrete spots with water (Control) or with 200 μg mL−1 laminarin (Lam), allowed to recover for 2 d, and protein was extracted from the infiltrated areas. Proteins were separated by SDS-PAGE, electrotransfered to a nylon membrane, and immunodetected with antibodies raised against the PR proteins families known as PR-1, PR-2, PR-3, and PR-5, respectively (Dorey et al., 1998). Data are representative of two independent experiments.