Figure 3.
Pathological changes and cell apoptosis in myocardial tissues. (A) Hematoxylin and eosin staining was performed to evaluate the pathological changes in myocardial tissues. Scale bars=50 µm. (B) TUNEL assay of infarcted border zone. Black arrows indicate apoptotic myocardial cells. Scale bars=50 µm. (C) Analyses of Bcl-2 and Bax by western blotting. β-actin served as an internal control. Values are presented as the mean ± standard deviation. **P<0.01, as indicated. TUNEL, terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; MI, myocardial infarction.