Figure 4. CdiI immunity is degraded by the Lon protease.

Average survival of exponentially growing Escherichia coli cells after 3 h of CIP treatment.

• A–C
  (A) ΔrelA, ΔspoT, (B) Δlon, (C) Δ lon, Δ sulA cells after 3 h of CIP treatment (n = 3 for Δ relA, Δ spoT, 4 for Δ lon and 3 for Δ lon, Δ sulA). Scales are logarithmic.
• D
  Immunoblot analysis of UPEC 536 CdiI‐HA protein (14.8 kDa) stability in the presence and absence of Lon protease before and after SHX treatment for 1 h. The lower panel shows the corresponding Coomassie‐stained SDS–PAGE gel as a volume loading control.

Data information: Error bars represent s.e.m. and n‐values indicate biological replicates.Source data are available online for this figure.