Figure 8. Population density‐dependent persister formation.

• A, B
  Average cfu/ml of exponentially growing MG1655 cells with empty (black bars), cdiBA‐CT‐I ^EC93 (white bars) and cdiBA‐CT‐I ^UPEC536 (grey bars) plasmids after treatment with CIP for 3 h. Cells at (A) low (˜10⁷) or (B) high (˜10⁸) confluence were taken from the same exponentially growing culture, centrifuged and resuspended in PBS with CIP (n = 5). Viable cfu/ml at 0, 1 and 3 h after centrifugation are shown. Error bars represent s.e.m. and n‐values indicate biological replicates. Statistical significance was determined using Mann‐Whitney U‐test. Scales are logarithmic. *P < 0.05, **P < 0.01.

Source data are available online for this figure.