Figure EV5. Structural centrosome aberrations trigger budding of mitotic cells through decreasing E‐cadherin junctions and increasing cellular stiffness.

Scheme shows that NLP‐induced structural centrosome aberrations favor budding of mitotic cells through two distinct pathways that are both necessary but not sufficient. On the one side, NLP‐induced centrosome aberrations induce a decreased of E‐cadherin junctions that can be rescued by partial inhibition of the Rac1 or Arp2/3 activities, which consequently prevents budding. On the other side, budding also requires an increased of cellular stiffness that can be restored by depolymerizing the actin network by a cytochalasin D treatment. Such a treatment can also prevent budding induced by NLP overexpression.