Figure 2.

Silencing RON impaired BC cell proliferation. Silencing RON impaired BC cell proliferation. Western blot analysis results indicated RON specific siRNA significantly downregulated the protein expression level. (A) The effect of RON siRNA on RON protein expression level using western blotting and (B) quantification of RON protein expression level in 5637 cells. (C) CCK-8 assay was performed following RON siRNA treatment. A reduction in absorbance was observed upon treatment with si-RON, when compared with si-Scr. Values are presented as the mean ± standard deviation from three independent experiments. *P<0.05 and **P<0.01, compared with si-Scr group. RON, macrophage stimulating 1 receptor; si-Scr, scramble small interfering RNA; si-RON, human RON sequence-specific duplex siRNA; OD, optical density.