Fig. 2.

Expression patterns of GmPIB1 in soybean. (A–C) GmPIB1 expression in soybean leaves in response to exogenous hormones: 100 μM MeJA, 2 mM SA, and ET treatment for 0, 1, 3, 6, 9, 12, and 24 h. Fourteen-day-old plants were used for treatments and analyses. Relative GmPIB1 transcript levels were compared with mock-treated plants at the same time point. Soybean GmEF1β was used as an internal control to normalize all data. Three biological replicates were averaged and statistically analysed using Student’s t-test (*P<0.05, **P<0.01). Bars indicate standard error of the mean. (D) GmPIB1 mRNA levels in various soybean plant tissues. Leaves, roots, and stems were harvested from 14-day-old plants. The experiment was performed on three biological replicates, each with three technical replicates. Bars indicate standard error of the mean. (E) GUS histochemical staining analysis of pGmPIB1:GUS. pGmPIB1:GUS and p35S:GUS transgenic soybean hairy roots were produced by A. tumefaciens-mediated transformation and treated with 100 μM MeJA, 2 mM SA, or ET for 6 h. GUS histochemical staining results 3 h after treatment are shown compared with roots treated with water. Bars, 1 cm. (F) GUS activity analysis of GmSPOD1 promoter expression. GUS activity was measured using a 4-methylumbelliferyl-D-glucuronide assay. The data represent the means ±SD of three independent experiments. (This figure is available in color at JXB online.)