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. 2018 Mar 22;69(10):2527–2541. doi: 10.1093/jxb/ery103

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — GmPIB1 forms a homodimer in yeast cells and in planta. (A) Yeast cells of strain Y₂H harboring pGBKT7-GmPIB1 and pGADT7-GmPIB1 plasmid combinations were grown on either SD/−Trp/−Leu or SD/−Trp/−Leu/−His/−Ade medium. Yeast cells carrying the pGBKT7-53 and pGADT7-SV40 plasmids were used as the positive control; yeast cells harboring the pGBKT7-Lam and pGADT7-SV40 plasmids were used as the negative control. (B) BiFC analysis of the interaction of GmPIB1 with itself. GmPIB1–YFP^N and GmPIB1–YFP^C were co-transfected into Arabidopsis protoplasts. The bright-field, YFP fluorescence (yellow), chlorophyll autofluorescence (red), and combined images were visualized under a confocal microscope 16 h after transfection. Bars, 10 μm. (This figure is available in color at JXB online.)