Table 2.
Primer Sequences for Quantitative PCR
| Gene | Protein | Forward (5′–3′) | Reverse (5′–3′) | Source |
|---|---|---|---|---|
| Csf2 | GM-CSF | TGAACCTCCTGGATGACATG | GTGTTTCACAGTCCGTTTCC | Bouaboula et al. (15) |
| Il1b | IL-1β | TTGACGGACCCCAAAAGATG | AGAAGGTGCTCATGTCCTCA | Bouaboula et al. (15) |
| Tnf | TNF-α | TCTCATCAGTTCTATGGCCC | GGGAGTAGACAAGGTACAAC | Bouaboula et al. (15) |
| Il6 | IL-6 | GTTCTCTGGGAAATCGTGGA | TGTACTCCAGGTAGCTATGG | Bouaboula et al. (15) |
| Il10 | IL-10 | ATGCAGGACTTTAAGGGTTACTTG | TAGACACCTTGGTCTTGGAGCTTA | Bouaboula et al. (15) |
| Nos2 | iNOS | ACATCGACCCGTCCACAGTAT | CAGAGGGGTAGGCTTGTCTC | Harvard primer bank (no. 146134510c2) |
| Arg1 | Arg 1 | CAGCACTGAGGAAAGCTGGT | CAGACCGTGGGTTCTTCACA | Newly designed |
| Dio1 | Deiodinase 1 | GAGCAGCCAGCTCTACGCGG | TGGGGAGCCTTCCTGCTGGT | van Zeijl et al. (16) |
| Dio2 | Deiodinase 2 | GCTTCCTCCTAGATGCCTACAA | CCGAGGCATAATTGTTACCTG | Kwakkel et al. (17) |
| Dio3 | Deiodinase 3 | CCAACTCTAGCAGTTCCGCA | GCCTCCCTGGTACATGATGG | Newly designed |
| Slc16a2 | MCT8 | GTGCTCTTGGTGTGCATTGG | GGGACACCCGCAAAGTAGAA | Bloise et al. (18) |
| Slc16a10 | MCT10 | TGATTCCCCTGTGCAGCGCC | CCACGTCGTAGGTGCCCAGC | Kwakkel et al. (4) |
| Thra1 | TRα1 | CATCTTTGAACTGGGCAAGT | CTGAGGCTTTAGACTTCCTGATC | Bakker (19) |
| Thra2 | TRα2 | CATCTTTGAACTGGGCAAGT | GACCCTGAACAACATGCATT | Bakker (19) |
| Thrb1 | TRβ1 | CACCTGGATCCTGACGATGT | ACAGGTGATGCAGCGATAGT | Boelen et al. (20) |
| Hprt | HPRT | GCAGTACAGCCCCAAAATGG | AACAAAGTCTGGCCTGTATCCAA | Sweet et al. (21) |
| Ppib | Cyclophillin B | GAGACTTCACCAGGGG | CTGTCTGTCTTGGTGCTCTCC | de Vries et al. (22) |
| Eef1a1 | EF1α1 | AGTCGCCTTGGACGTTCTT | ATTTGTAGATCAGGTGGCCG | Kwakkel et al. (4) |
| Rplp0 | RPL0 | GGCCCTGCACTCTCGCTTTC | TGCCAGGACGCGCTTGT | Bloise et al. (18) |
Abbreviations: GM-CSF, granulocyte macrophage colony–stimulating factor; iNOS, inducible nitric oxide; TNF, tumor necrosis factor.