SPARC and β-actin mRNA expression in (A) 7 gastric cancer cell lines and (B) clinical samples, as determined by reverse-transcription polymerase chain reaction. The product size of SPARC and β-actin was 126 and 171 base pairs, respectively. SPARC, secreted protein, acidic and cysteine-rich; p, positive control; n, negative control; T, tumor; N, adjacent normal mucosa.