Figure 2.

Cell colony formation assay. MKN-45 cells were treated with glabridin, 5-FU, glabridin plus 5-FU or control medium. The number of cell clones was counted under the microscope (>50 clones validated), and four separate experiments were performed for average. Randomly select 5 different fields to be counted in each well, and four separate experiments were performed for average. Data was obtained from at least three independent experiments. *P<0.05 (vs. control group) or #P<0.05 (glabridin +5-FU vs. 5-FU) was considered to indicate a statistically significant difference. 5-FU, 5-fluorouracil.