The signal transducer and activator of transcription 3-cyclooxygenase-2 signaling pathway serves a function in NaHS-induced increase in cell viability in PLC/PRF/5 cells. (A) PLC/PRF/5 cells were treated with different concentration (100, 200, 300, 400 and 500 µmol/l) of NaHS. (B) Treatment of PLC/PRF/5 cells with 500 µmol/l NaHS for the indicated times (12, 24, 36 and 48 h). (C) co-treatment of PLC/PRF/5 cells with 500 µmol/l NaHS and different doses of AG490 (1, 10, 20 and 30 µmol/l) for 24 h. (D) PLC/PRF/5 cells were co-treated with 500 µmol/l NaHS and NS-398 (0.01–0.5 µmol/l) for 24 h. Cell viability was detected by Cell Counting Kit-8 assay. Data are presented as the mean ± standard error of the mean (n=5). *P<0.05, **P<0.01 vs. the control group. +P<0.05, ++P<0.01 vs. with the NaHS-treated group. Con, control.