Skip to main content
. 2018 Mar 2;15(5):6562–6570. doi: 10.3892/ol.2018.8154

Figure 4.

Figure 4.

The STAT3-COX2 signaling pathway participates in NaHS-induced migration in PLC/PRF/5 cells. (A) Exposure of PLC/PRF/5 cells for the indicated times (1, 3, 6, 9, 12 and 24 h) to 500 µmol/l NaHS; (B) the rate of MMP-2/GAPDH; (C) co-treatment of PLC/PRF/5 cells with 500 µmol/l NaHS and 30 µmol/l AG490 for 24 h; (D) the rate of MMP-2/GAPDH; (E) PLC/PRF/5 cells were co-treated with 500 µmol/l NaHS and 20 µmol/l NS-398 for 24h; (F) the rate of MMP-2/GAPDH; (G) PLC/PRF/5 cells were co-treated with 500 µmol/l NaHS and 30 µmol/l AG490, or 20 µmol/l NS-398 for 24 h. (A-F) The expression levels of MMP-2 were semiquantified by western blot assay. (C, D and F) Densitometric analysis of the p-STAT3 expression levels shown in panels A-C, respectively. (G) Cell migration was evaluated by Transwell migration assay, and the migration rate was calculated under a fully automated inverted microscope. Data are presented as the mean ± standard error of the mean (n=3). **P<0.01 vs. the control group. ++P<0.01 vs. the NaHS-treated group. MMP-2, matrix metalloproteinase-2; Con, control.