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. 2018 Mar 19;15(5):7649–7654. doi: 10.3892/ol.2018.8294

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Copyright: © Tang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — miR-874 directly targets CDK9 in osteosarcoma cells. (A) Sequence alignment of miR-874 and 3′UTR of CDK9 using mirco-RNA.org. (B) Luciferase reporter assay. HEK293T cells were transiently co-transfected with Wt/Mut 3′UTR of CDK9 with miRNAs as indicated. (C) Quantitative polymerase chain reaction assay revealed the effects of miR-874 on the expression levels of CDK9 mRNA. (D) Western blot analysis revealed the effects of miR-874 on the expression levels of CDK9 miRNA in cells. Data are presented as the mean ± standard deviation from three independent experiments. *P<0.01 vs. the control group. CDK9, cyclin-dependent kinase 9; UTR, untranslated region; Mut, mutant; Wt, wild-type; miRNA, microRNA; miR-874, microRNA-874.