Table II.
HSR | HSR + PA | HSC | HSC + PA | |||||
---|---|---|---|---|---|---|---|---|
Variables | Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM |
Final body weight, g | 171 | 4 | 176 | 4 | 178 | 1 | 183 | 2 |
Food intake, g/12 day | 174a | 4 | 177a,b | 4 | 188b,c | 2 | 193b | 3 |
Liver | ||||||||
Weight, g/100 g body wt. | 4.64a | 0.10 | 4.71a | 0.12 | 5.84b | 0.13 | 5.70b | 0.06 |
Total lipids, mg/g liver | 64.2a | 3.6 | 57.8a | 1.2 | 106.2b | 6.2 | 62.5a | 1.4 |
Triglyceride, mg/g liver | 17.5b | 1.9 | 12.0a | 0.3 | 51.6c | 5.6 | 23.2b | 1.6 |
Cholesterol, mg/g liver | 3.22 | 0.29 | 3.13 | 0.15 | 3.56 | 0.15 | 3.04 | 0.13 |
G6PD activity, mU/mg protein | 34.1a | 5.4 | 36.7a | 1.7 | 69.1b | 7.8 | 53.1a,b | 6.6 |
ME activity, mU/mg protein | 28.9a | 2.9 | 32.1a | 2.8 | 50.6b | 5.6 | 35.7a,b | 3.5 |
G6PD expression | 1.00a | 0.12 | 0.89a | 0.10 | 1.71b | 0.09 | 1.12a | 0.09 |
ME1 expression | 1.00a,b | 0.06 | 0.72a | 0.05 | 2.08c | 0.07 | 1.09b | 0.06 |
FAS expression | 1.00a | 0.11 | 0.88a | 0.16 | 2.79b | 0.78 | 2.21a,b | 0.06 |
Mean values with their SEM; n=6.
Mean values with unlike superscript letters were significantly different, P<0.05 (Tukey post hoc test for body weight, food intake, liver weight, hepatic cholesterol, hepatic ME activity, hepatic gene expression of lipogenic enzymes; Steel-Dwass test for liver total lipids, hepatic triglyceride and hepatic G6PD activity). HSR, high-starch; HSC, high-sucrose; PA, sodium phytate; G6PD, glucose-6-phosphate dehydrogenase; ME, malic enzyme; FAS, fatty acid synthetase; SEM, standard error of the mean.