Table III.
HSR | HSR + PA | HSC | HSC + PA | |||||
---|---|---|---|---|---|---|---|---|
Variables | Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM |
Feces | ||||||||
Weight, g/3 days | 3.89a,b | 0.14 | 3.96a,b | 0.13 | 3.54a | 0.07 | 4.11b | 0.14 |
Microbiota,% of total bacteria | ||||||||
Lactobacillus spp. | 4.76a,b | 1.81 | 7.20b | 2.11 | 0.15a | 0.03 | 9.29b | 3.01 |
Bifidobacterium spp. | 0.077 | 0.044 | 0.153 | 0.090 | 0.013 | 0.009 | 0.006 | 0.001 |
Clostridium coccoides | 7.32a | 1.39 | 3.56b | 0.61 | 7.17b | 1.26 | 0.02a | 0.01 |
Bacteroides | 15.9a,b | 2.1 | 14.7a | 2.9 | 17.1a,b | 2.0 | 27.4b | 4.3 |
Cecum | ||||||||
Tissue weight, g/100 g body wt. | 0.380b | 0.029 | 0.335a,b | 0.011 | 0.264a | 0.017 | 0.322a,b | 0.022 |
Cecal digesta | ||||||||
Weight, g | 2.47a | 0.12 | 3.22b | 0.19 | 1.93a | 0.12 | 2.25a | 0.11 |
pH | 6.90b | 0.18 | 6.22a | 0.05 | 7.62c | 0.15 | 6.92b | 0.09 |
Microbiota, % of total bacteria | ||||||||
Lactobacillus spp. | 1.66a,b | 0.79 | 1.88b | 0.71 | 0.04a | 0.01 | 0.50b | 0.06 |
Bifidobacterium spp. | 0.0077a,b | 0.0043 | 0.0277a,b | 0.0083 | 0.0024a | 0.0003 | 0.0060b | 0.0015 |
Clostridium coccoides | 8.34b | 1.69 | 2.36a,b | 0.78 | 5.39b | 0.79 | 0.48a | 0.36 |
Bacteroides | 30.2 | 1.8 | 45.7 | 8.8 | 30.3 | 2.0 | 53.4 | 13.9 |
Organic acids (µmol/total digesta) | ||||||||
Succinate | 2.1a | 0.8 | 129.3b | 12.3 | 0.9a | 1.3 | 59.2b | 18.3 |
Lactate | 2.5 | 1.2 | 7.7 | 2.7 | 1.3 | 0.4 | 2.0 | 0.6 |
Acetate | 93.4a,b | 12.4 | 129.9b | 17.7 | 69.2a | 3.5 | 67.4a,b | 6.6 |
Propionate | 34.6b | 3.5 | 39.4a,b | 8.5 | 23.6a,b | 1.5 | 18.0a | 1.7 |
n-Butyrate | 34.5 | 7.2 | 34.7 | 3.5 | 25.9 | 1.2 | 45.2 | 25.8 |
Total organic acids | 165.3a,b | 24.2 | 341.2c | 23.3 | 121.2a | 4.9 | 190.7b | 25.3 |
Mean values with their SEM; n=6.
Mean values with unlike superscript letters were significantly different, P<0.05 (Tukey post hoc test for fecal weight, fecal Bacteroides, cecum weight, cecal digesta weight, cecal pH; Steel-Dwass test for fecal Lactobacillus spp., fecal Clostridium coccoides, fecal Bifidobacterium spp., cecal microbiota and cecal organic acids). HSR, high-starch; HSC, high-sucrose; PA, sodium phytate; SEM, standard error of the mean.