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. 2018 Mar 16;8(5):466–474. doi: 10.3892/br.2018.1079

Table IV.

Fecal and cecal microbiota and cecal organic acids in rats fed an HSC diet with or without MI or PA (experiment 2).

HSC HSC + PA HSC + MI



Variables Mean SEM Mean SEM Mean SEM
Final body weight, g 175 4 179 1 178 4
Food intake, g/12 days 183 4 183 2 185 5
Liver
  Weight, g/100g body wt. 5.83a 0.08 5.52a,b 0.08 5.31b 0.12
Feces
  Weight, g/3 days 3.51 0.08 3.89 0.18 3.60 0.19
Cecum
  Tissue weight, g/100 g body wt. 0.245 0.005 0.291 0.011 0.270 0.017
  Cecal digesta weight, g 1.73a 0.05 2.08b 0.09 1.85a,b 0.02
    pH 7.65c 0.09 6.87a 0.05 7.10b 0.04
Microbiota, % of total bacteria
    Lactobacillus spp. 0.006a 0.002 0.171b 0.143 0.434b 0.325
    Bifidobacterium spp. 0.0031a 0.0005 0.0197b 0.0098 0.0178a,b 0.0098
    Clostridium coccoides 4.54b 0.50 1.02a 0.51 4.30b 0.36
    Bacteroides 31.9 1.5 52.6 11.3 26.4 1.9
Organic acids, µmol/total digesta
  Succinate 14.4a 3.6 36.7b 4.3 9.6a 2.5
  Lactate 3.9 2.0 3.8 1.8 4.6 0.7
  Acetate 51.2 2.6 55.0 3.6 52.7 3.6
  Propionate 17.9 1.8 18.1 1.4 18.9 0.6
  n-Butyrate 18.8b 1.2 21.2a,b 1.0 23.4a 1.8
  Total organic acids 106.2a 7.2 134.8b 4.8 109.2a,b 6.8

Mean values with their SEM; n=6.

a-c

Mean values with unlike superscript letters were significantly different, P<0.05 (Tukey post hoc test for body weight, food intake, liver weight, fecal weight, cecal pH, cecal Clostridium cocoides and cecal organic acids; Steel-Dwass test for cecum weight, cecal digesta weight, cecal Bifidobacterium spp., cecal Lactobacillus spp. and Bacteroides). HSR, high-starch; HSC, high-sucrose; PA, sodium phytate; MI, myo-inositol; SEM, standard error of the mean.