Table 1.
Polymerase chain reaction (PCR) primers and reacting conditions.
Strain | Primer Sequence | PCR Condition | Ref. | |
---|---|---|---|---|
L. lactis subsp. Lactis | sense | 5′-CGTTATGGATTTGATGGATATAAAGC-3′ | 94 °C 9 min, 94 °C 30 s, 50 °C 30 s, 72 °C 60 s × 45 cycles, 72 °C 7 min | [27] |
antisense | 5′-ACTCTTCTTAAGAACAAGTTTAACAGC-3′ | |||
L. lactis subsp. Cremoris | sense | 5′-CGTTATGGATTTGATGGATATAAAGC-3′ | 94 °C 9 min, 94 °C 30 s, 50 °C 30 s, 72 °C 60 s × 45 cycles, 72 °C 7 min | |
antisense | 5′-ACTCTTCTTAAGAACAAGTTTAACAGC-3′ | |||
L. lactis subsp. lactis biovar diacetylactis | sense | 5′-CTTCGTTATGATTTTACA-3′ | 94 °C 30 s, 94 °C 30 s, 46 °C 60 s, 72 °C 60 s × 30 cycles, 72 °C 2 min | [28,29] |
antisense | 5′-AATATCAACAATTCCATG-3′ | |||
Leuconostoc mesenteroides subsp. Cremoris (Genus-specific primers for Leuconostoc) | sense | 5′-AACTTAGTGTCGCATGAC-3′ | 94 °C 5 min, 94 °C 60 s, 60 °C 60 s, 72 °C 60 s × 30 cycles, 72 °C 10 min | [30] |
antisense | 5′-AGTCGAGTTACAGACTACAA-3′ | |||
Lactobacillus plantarum | sense | 5′-CCGTTTATGCGGAACACCTA-3′ | 94 °C 3 min, 94 °C 30 s, 54 °C 10 s, 72 °C 30 s × 30 cycles, 72 °C 5 min | [31] |
antisense | 5′-TCGGGATTACCAAACATCAC-3′ | |||
Lactobacillus casei | sense | 5-TGCACTGAGATTCGACTTAA-3 | 94 °C 3 min, 94 °C 45 s, 45 °C 45 s, 72 °C 60 s × 30 cycles, 72 °C 5 min | [32] |