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. 2018 Apr 2;7(4):67. doi: 10.3390/jcm7040067

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Differentiation of engrafted human Sk-34 cells into Schwann cells and perineurial cells, and the number of blood vessels in the bridging conduit. (A) Transplanted human Sk-34 cells (pink nuclei) expressed a newly formed Schwann cell marker p75 (green); (B) Transplanted human Sk-34 cells (pink nuclei) also formed new perineurium (green) showing differentiation into perineurial cells; (C) Typical staining of CD31 (green) as evidence of the number of blood vessels in the conduit. Blue staining in each panel is the nuclear staining of DAPI. P1 = position 1, P2 = position 2. Bars in A and B = 50 μm, C = 100 μm; (D) Statistical comparison of the formation of blood vessels in the conduit. There is no difference in the proximal Portion 1 between NE and E group, but a significantly higher value in (E) was observed in the distal end Portion 3. * p < 0.05.