Figure 1.

TMZ resistance promotes the formation of cancer-initiating cells and upregulates FAP-α expression in glioblastoma (n=3). (A) MTT for cell viability in U251MG and U251MG-R cells. Cells were untreated or treated with TMZ. (B) Sphere growth for U251MG and U251MG-R cells. Scale bar, 200 µm. (C) Western blotting for STAT3, MDM2, CD133 and MET in U251MG and U251MG-R cells. β-actin was the loading control. (D) Western blotting for FAP-α in U251MG and U251MG-R cells. β-actin was the loading control. R, resistant; TMZ, temozolomide; FAP-α, Fibroblast activation protein α; STAT3, Signal transducer and activator of transcription 3; MDM2, Mouse double minute 2 homolog; CD133, Prominin-1; MET, Tyrosine-protein kinase Met.